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. 2017 Oct 16;8:556. doi: 10.3389/fneur.2017.00556

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Copyright © 2017 Qiu, Wang, Wu, Wan, Cheng, Wu and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Low dose of apelin-36 attenuates cerebral I/R injury-induced apoptosis in rats. (A) The forebrains of rats were dissected into 5 µm coronal slices and terminal deoxynucleotidyltransferase-mediated DUTP-biotin nick end labeling (TUNEL) assay was performed. TUNEL-positive cells are indicated by green color and the nuclei were counterstained with DAPI (blue). (B) Quantification of TUNEL assay. Apoptosis ratio was calculated by dividing the number of apoptotic cells by the total cell number. Apelin represents apelin-36. Values represent mean ± SEM. N = 5, *p < 0.05 by ANOVA followed by Tukey test.