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Concentration and specific activity of recombinant creatinase which expressed in different induced temperatures (22, 28 and 37°C)
| Samples | Absorbance at 595 nm | Sample concentration (mg/ml) | Absorbance at 435 nm | Activity (unit/ml) | Specific activity (unit/mg) |
|---|---|---|---|---|---|
| Recombinant creatinase expressed at 22°C | 1.194 | 0.437 | 1.18 | 11.02 | 25.23 |
| Recombinant creatinase expressed at 28°C | 1.848 | 0.727 | 1.42 | 13.27 | 18.53 |
| Recombinant creatinase expressed at 37°C | 1.4 | 0.438 | 1.24 | 11.58 | 26.54 |
