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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Osteoarthritis Cartilage. 2017 Aug 8;25(11):1880–1889. doi: 10.1016/j.joca.2017.07.023

Fig. 2.

Fig. 2

Age-related changes in ATG-5 and LC3 expression and cellularity. Immunohistochemistry for ATG-5 and LC3 were performed on mouse knee joint sections. (A) Representative images of knee joints from 6- and 30-month-old mice stained with ATG-5, LC3, and Hematoxylin (H), showing the anterior (A) and posterior meniscus, femur (F) and tibia (T). Original magnification = 10X, Scale bar = 100 μm. Quantitative analysis of ATG-5 (B) and LC3 (C) positive cell density per 100 μm2 area. Hematoxylin (H) stained sections were analyzed by total cell density per 100-μm2 area (D). Results show a significant decrease in ATG-5 and LC3 cell number and reduced cellularity. Box and whisker plots (5 mice per group); * = P < 0.05 versus 6-month-old mice.