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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Osteoarthritis Cartilage. 2017 Aug 8;25(11):1880–1889. doi: 10.1016/j.joca.2017.07.023

Fig. 4.

Fig. 4

Autophagic response following meniscus injury. Two-month-old C57BL/6J transgenic mice were subjected to surgical transection of the medial meniscotibial ligament and medial collateral ligament in the right knee. The left knee was not subjected to surgery but was used as a control. The knee joints were analyzed by Safranin-O staining and immunohistochemistry for ATG-5. (A) Representative images of the control and injured mice knee joints stained with Safranin-O and ATG-5, showing the anterior (A) and posterior meniscus, femur (F) and tibia (T). Original magnification = 10X, Scale bar = 100 μm. (B) Quantitative analysis of ATG-5 positive cell density per 100 μm2 area. Results show significant increase in ATG-5 expressing cells in the anterior menisci. Box and whisker plots (5 mice per group); * = P < 0.05 versus control.