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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Endocr Relat Cancer. 2017 Sep 19;24(11):579–591. doi: 10.1530/ERC-17-0229

Figure 8. α-KG reverses stem-like phenotype in vitro.

Figure 8

A. SDHD knockdown NthyOri 3.1 cells with aKG (0.1 mM) treatment for 3 days were subjected to FACS analysis for measurement of ALDH activity. The percentage of ALDHhigh cells from single experiment is shown in each panel. Quantification of three independent experiment measuring % ALDH positive cells gated according to DEAB treated negative control is shown on right (statistical comparison is to the untreated Sh-Ctrl). B. DNA methylation levels of NthyOri 3.1 cells after treatment with 1mM α-KG for 3 days. Graphs represents mean ± SD of five biological replicates. C. Migration in thyroid cell lines treated with aKG (0.1 mM) at 12 hours performed by wound healing assays. Graph represents data from three independent experiments. Error bars represents standard deviation (SD). Statistical analyses were performed by two-tailed student’s t-test for ALDH assay and migration, and by Mann Whitney test in case of DNA methylation. (*P value ≤ 0.05, **P value ≤ 0.01).