Effect of stroke on depressive-like behaviors in MS P2X4R KO mice. (A) At baseline, both male and female mice showed no difference in the consumption of sucrose. However, MS P2X4R KO mice showed reduced consumption of sucrose pellet at post-stroke (B) day 15 [male (KO = 10, WT = 7) and female (KO = 8, WT = 9)] and (C) day 30 [males (KO = 5, WT = 4) and females (KO = 3, WT = 4)]. (D) Consistent with the SCT data, these mice (both sexes) [Female KO = 8 WT = 9; Male KO = 7, WT = 10)] showed a significant increase in depressive behavior (immobility duration) measured by the TST after 29 days of stroke (*p < 0.05; KO vs. WT; two-tailed Student’s t-test).