Figure 5.

Iron load phenotype in the lung of WT and Hepc KOliv mice: Plasma iron in μM (A) and lung iron in μg/g wet tissue (B). Pulmonary Tfrc, and Slc40A1 mRNA levels relative to Ppia analyzed by real-time PCR. Changes are expressed relative to WT mice (C). L-ferritin and ferroportin (D) protein levels analyzed by WB using proteins from cytosolic and membrane enriched-fractions, respectively. Representative images of BAL (bronchoalveolar lavages) cytospin slides obtained from WT and Hepc KOliv mice and stained with Perls' Prussian blue (E).The set of results obtained with WT and Hepc KOliv mice were reproduced using another serie of animals. Error bars represent SEM for n = 3–4 mice in each group. Statistical significance is indicated by asterisks (^*p < 0.05, ^**p < 0.01, ^***p < 0.001).