Table 2.

Box–Behnken experimental design matrix employed by DesignExpert^TM V.10 with experimental results to predict optimum concentrations of medium components.

Run	Design matrix			Dry biomass (g/L)
	A	B	C
1	1000	3.2	26.5	4.16
2	1500	1.2	3.0	4.26
3	1000	2.2	50.0	4.07
4	2000	1.2	26.5	4.19
5	2000	2.2	50.0	4.21
6	2000	3.2	26.5	4.27
7	1500	1.2	50.0	4.49
8	2000	2.2	3.0	4.18
9	1500	2.2	26.5	5.33
10	1500	2.2	26.5	5.27
11	1500	3.2	3.0	4.33
12	1500	2.2	26.5	5.39
13	1000	1.2	26.5	4.30
14	1000	2.2	3.0	4.09
15	1500	2.2	26.5	5.29
16	1500	3.2	50.0	4.17
17	1500	2.2	26.5	5.33