Table 3.
Effect of perturbations of ATP- and NADPH-consuming enzyme encoding genes on putrescine production in C. glutamicum PUT-ALE.
| Gene targeted | Gene encoding enzyme/protein | OD600 | Putrescine (mM) | Ratio |
|---|---|---|---|---|
| ATP-consuming | ||||
| Control | 16.83 ± 0.18 | 103.66 ± 3.29 | 1.00 | |
| carB | Carbamoyl-phosphate synthase large subunit [EC6.3.5.5] | 17.27 ± 0.20 | 109.00 ± 4.36 | 1.05 |
| thrB1 | Homoserine kinase [EC 2.7.1.39] | 17.02 ± 0.20 | 105.05 ± 0.45 | 1.01 |
| coaA | Pantothenate kinase [EC 2.7.1.33], | 16.56 ± 0.51 | 110.18 ± 1.32 | 1.06 |
| glnA | Glutamine synthetase [EC 6.3.1.2] | 15.63 ± 0.06 | 86.44 ± 5.17 | 0.86 |
| nadD | Nicotinate-nucleotide adenylyltransferase [EC 2.7.7.18] | 16.71 ± 0.18 | 95.68 ± 0.93 | 0.92 |
| hemH | Phosphoribosylaminoimidazole-succinocarboxamide synthase [EC 6.3.2.6], | 16.55 ± 0.35 | 86.74 ± 8.33 | 0.84 |
| xylB | Xylulokinase [EC 2.7.1.17] | 16.62 ± 0.42 | 108.88 ± 0.07 | 1.05 |
| guaA | GMP synthase (glutamine-hydrolysing) [EC:6.3.5.2], | 17.25 ± 0.24 | 82.71 ± 1.40 | 0.80 |
| accBC | Acyl-CoA carboxylase | 18.26 ± 0.42 | 80.20 ± 0.55 | 0.77 |
| accDA | Acetyl-CoA carboxylase beta subunit [EC 6.4.1.2] | 17.36 ± 0.10 | 109.24 ± 0.04 | 1.05 |
| purL | Phosphoribosylformylglycinamidine synthase [EC 6.3.5.3] | 16.60 ± 0.54 | 108.86 ± 0.59 | 1.05 |
| purQ | Phosphoribosylformylglycinamidine synthase [EC 6.3.5.3] | 17.91 ± 0.38 | 103.78 ± 5.31 | 1.00 |
| panC1 | Pantoate-beta-alanine ligase [EC 6.3.2.1] | 18.98 ± 1.34 | 107.24 ± 0.98 | 1.03 |
| panC2 | Pantoate-beta-alanine ligase [EC 6.3.2.1] | 17.98 ± 0.58 | 113.94 ± 2.98 | 1.10 |
| pknG | Serine/threonine protein kinases [EC 2.7.11.1] | 17.59 ± 0.35 | 109.57 ± 1.21 | 1.06 |
| NADPH-consuming | ||||
| Control | 19.80 ± 0.30 | 108.99 ± 2.51 | 1.00 | |
| pobA | p-hydroxybenzoate 3-monooxygenase [EC 1.14.13.2] | 15.90 ± 0.24 | 105.36 ± 2.53 | 0.93 |
| aldH | 2,5-dioxopentanoate dehydrogenase [EC 1.2.1.26] | 16.07 ± 0.15 | 92.65 ± 3.83 | 0.88 |
| fabG1 | 3-oxoacyl-[acyl-carrier protein] reductase [EC 1.1.1.100], | 16.08 ± 0.26 | 108.96 ± 0.79 | 0.96 |
| adhC | Maleylacetate reductase [EC 1.3.1.32] | 15.62 ± 0.16 | 101.45 ± 2.38 | 0.93 |
| gor | Dihydrolipoamide dehydrogenase/glutathione oxidoreductase and related enzymes, [EC 1.8.1.7] | 15.52 ± 0.35 | 94.92 ± 3.05 | 0.87 |
| dxr | 1-deoxy-D-xylulose-5-phosphate reductoisomerase [EC 1.1.1.267] | 15.36 ± 0.50 | 123.18 ± 0.55 | 1.13 |
| asd | aspartate-semialdehyde dehydrogenase [EC 1.2.1.11] | 15.54 ± 0.18 | 99.7 ± 1.48 | 0.91 |
| proA | Glutamate-5-semialdehyde dehydrogenase [EC 1.2.1.41] | 16.21 ± 0.19 | 98.51 ± 0.87 | 0.90 |
| NCgl2558 | Transcriptional regulators | 15.87 ± 0.12 | 99.06 ± 1.92 | 0.87 |
| thyX | Thymidylate synthase (FAD) [EC 2.1.1.148] | 19.77 ± 0.48 | 103.71 ± 3.55 | 0.95 |
| aroE | Shikimate dehydrogenase [EC 1.1.1.25], | 16.74 ± 0.06 | 129.29 ± 1.76 | 1.19 |
| sir | Sulfite reductase (ferredoxin) [EC 1.8.7.1] | 17.82 ± 0.30 | 106.87 ± 2.02 | 0.98 |
| NCgl0503 | Aldo/keto reductases | 15.18 ± 2.58 | 110.53 ± 3.30 | 1.01 |
| ddh | diaminopimelate dehydrogenase [EC 1.4.1.16] | 15.93 ± 0.09 | 98.82 ± 2.68 | 0.91 |
| ilvC | Ketol-acid reductoisomerase [EC 1.1.1.86] | 16.32 ± 0.30 | 102.49 ± 3.71 | 0.94 |
| qor | NADPH:quinone reductase and related Zn-dependent oxidoreductases [EC 1.6.5.5] | 16.83 ± 0.27 | 108.78 ± 0.34 | 0.98 |
| trxB | Thioredoxin reductase (NADPH) [EC 1.8.1.9] | 17.16 ± 0.36 | 131.12 ± 0.88 | 1.20 |
| NCgl0200 | NADPH:quinone reductase and related Zn-dependent oxidoreductases | 16.26 ± 0.24 | 109.30 ± 0.76 | 0.99 |
Data represent the average of three replicates and error bars represent standard deviation.