Skip to main content
. 2017 Oct 17;9:337. doi: 10.3389/fnagi.2017.00337

Figure 1.

Figure 1

Effect of cilostazol (CSZ) on viability and staining with annexin V and Hoechst33342 in amyloid β (Aβ)-stimulated SH-SY5Y cells. We evaluated the effect of Aβ on cell viability using MTT assay and stained with annexin V and Hoechst33342. (A) Cell viability of SH-SY5Y cells treated with Aβ and CSZ for 20 h. (B) The effect of Aβ with or without CSZ on cell viability of SH-SY5Y cells. (C) The effect of Aβ and CSZ with or without the inhibitor of MAPK/ERK kinase1/2 (MEK1/2) (PD98059) or p38 mitogen-activated protein kinases (MAPK) (SB202190) on cell viability of SH-SY5Y cells. Cells were observed under a phase-contrast microscope (D–H) and a fluorescence microscope (I–M). Images (D,I) untreated SH-SY5Y cells; images (E,J) SH-SY5Y cells treated with Aβ; and images (F,K): SH-SY5Y cells treated with Aβ + CSZ; images (G,L) SH-SY5Y cells treated with Aβ + CSZ + inhibitor of MEK1/2; images (H,M) SH-SY5Y cells treated with Aβ + CSZ + inhibitor of p38 MAPK. Values are expressed as mean + SEM. #Compared vs. respective controls (p < 0.01). *Compared vs. Aβ-treated cells (p < 0.05). $Compared vs. Aβ + CSZ-treated cells (p < 0.05). $$Compared vs. Aβ + CSZ-treated cells (p < 0.01).