Table 1.
Numerical and statistical analysis of ribosomal subunit peaks.
| Ratio subunits vs. polysome (I) |
||||
|---|---|---|---|---|
| 30S | 50S | 70S∗ | ||
| ASet 1 | Vector | 1.06 | 1.42 | 6.76 |
| Fic1 | 1.08 | 1.52 | 3.15 | |
| Fic2 | 1.47 | 1.70 | 2.64 | |
| Fic1 + Fic2 | 0.86 | 1.26 | 3.07 | |
| BSet 2 | Vector | 1.06 | 1.40 | 3.21 |
| Fti3 | 1.16 | 1.34 | 3.38 | |
| Fic3 | 1.94 | nd | 11.84 | |
| Fti3 + Fic3 | 1.10 | 1.67 | 1.67 | |
| Vector | Fic2 | p-Values∗∗ | ||
| Statistic (n = 7) | 30S | 1.10 ± 0.02 | 1.50 ± 0.19 | 0.0005 |
| 50S | 1.49 ± 0.11 | 2.03 ± 0.38 | 0.0137 | |
A,Bsee Figure 8; Numbers represent ratios of 30S or 50S subunit versus first polysome peak (I) values. Numbers were calculated based on XSpan generated “Heights” (see section “Materials and Methods”); nd, 50S peaks are not separated enough from 70S peaks for numerical analysis with XSpan; ∗Height was generated by XSpan after extrapolation of clipped curves. ∗∗p-Values were calculated from seven profiles using the paired Student’s t-test.