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. 2017 Oct 17;8:2015. doi: 10.3389/fmicb.2017.02015

FIGURE 6.

FIGURE 6

SDS–PAGE analysis of the purified CopRHis6 and specific binding of CopRHis6 to the predicted binding sites upstream of the copB gene. (A) SDS–PAGE analysis of the purified CopRHis6. Lanes 1 and 2, L. lactis NZCK and NZCopRHis6 with 10 ng ml-1 nisin induction; lanes 3 and 4, purified products of NZCK and NZCopRHis6. (B) EMSAs showing the specific interaction of CopR and the predicted binding sites upstream of copB. Binding reactions consisted of the following: lane 1, 20 fmol labeled probe alone; lane 2, 20 fmol labeled probe, and 2.5 μg CopRHis6; lane 3, 20 fmol labeled probe, 4 pmol unlabeled probe, and 2.5 μg CopRHis6.