FIGURE 8.

EMSAs showing the interaction of CopR and the predicted binding sites upstream of copB in the presence of different concentrations of Cu²⁺. Binding reactions consisted of the following: lane 1, 20 fmol labeled probe alone; lane 2, 20 fmol labeled probe and 2.5 μg CopRHis₆; lane 3, 20 fmol labeled probe and 2.5 μg CopRHis₆ and 5 μM Cu²⁺; lane 4, 20 fmol labeled probe, 2.5 μg CopRHis₆, and 10 μM Cu²⁺; lane 5, 20 fmol labeled probe, 2.5 μg CopRHis₆, and 20 μM Cu²⁺; and lane 6, 20 fmol labeled probe, 2.5 μg CopRHis₆, and 50 μM Cu²⁺.