Table 3. Particle size and polydispersity index of CS nanoparticles .

No.	Factors			Experimental result
	CS Conc.(%)	TPP Conc.(%)	Stirring speed(rpm)	Particle size (nm)(mean±SD)	Polydispersity index(mean±SD)
1	0.2	0.3	6000	5.77±117.33	0.208±1.290
2	0.2	0.45	9000	2.64±112	0.056±1.110
3	0.2	0.675	13500	45.13±160	0.225±1.49
4	0.3	0.3	9000	15.00±94	0.016±0.785
5	0.3	0.45	13500	92.66±623.33	0.027±0.710
6	0.3	0.675	6000	2.89±111.67	0.058±1.103
7	0.45	0.3	13500	29.51±338	0.059±0.717
8	0.45	0.45	6000	13.32±71.67	0.057±0.705
9	0.45	0.675	9000	83.74±159.33	0.169±1.001

Abbreviations: CS Conc., chitosan concentration; TPP Conc. tripolyphosphate concentration. Data are presented as means ± standard deviations (n = 3).