Figure 5.

Experiment 3, (A–D) immunofluorescent staining of the VPM/VPL thalamus of a rat 4 weeks after infusion of AAV8-hSyn-hM4D(Gi)-mCherry. The representative brain slice was collected from a rat whose whisker pad was injected with control MeWo cells and given an IP injection of vehicle (0.5% saline). Arrows point to NeuN positive cells expressing mCherry, arrowhead points to NeuN positive cell that did not express mCherry. Nuclei were stained blue with Hoechst 33342. Bar equals 50 micrometers. (E) Histogram of blinded cell counts in circular field 0.5 mm² in area, counts were completed in randomly chosen fields adjacent to the injection site in several thalamic regions; the VPM, VPL and reticular (Rt) thalamic nuclei, as well as the zona incerta (ZI). Asterisk indicates p < 0.05 when comparing the NeuN+/mCherry positive cells in the VPM/VPL to the NeuN+/mCherry positive cells in the Rt and ZI. n = 3 for the control/vehicle and control/CNO groups and n = 4 for the VZV/vehicle and VZV/CNO groups. Values are the mean and SEM.