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. 2017 Oct 8;2017:7658970. doi: 10.1155/2017/7658970

Figure 1.

Figure 1

Design of the recombinant transfer vector for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins and confirmation of the recombinant transfer vector and baculovirus bacmids. M indicates the DNA size marker. (a) Sites of insertion of the coding sequences for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins in the baculovirus transfer vector pFastBac 1. (b) The recombinant transfer vectors pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 were digested with the restriction endonucleases BamHI and HindIII and subjected to agarose gel electrophoresis. Lanes 1, 3, and 5: undigested plasmids. Lanes 2, 4, and 6: pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 digested with BamHI and HindIII, respectively. (c) The recombinant bacmids rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2 were amplified by PCR with the M13 universal primers, and the PCR products were subjected to agarose gel electrophoresis. Lanes 1, 2, and 3: PCR products from rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2, respectively.