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Verification of the expression level of genes related to UPR and ER press. NES-GFP iPSC-derived NPCs were treated with propofol (0, 20, 50, 100, or 300 μM) for 6 h. The mRNA expression level of ATF4, CEBPB, DDIT3, and TRIB3 was evaluated by microarray (a, b, c, d) and qRT-PCR (e, f, g, h). The qRT-PCR results were normalized to the GAPDH mRNA level. All data are presented as mean ± SD (n = 3; ∗p < 0.05; ∗∗∗p < 0.001, Student's t-test).
