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. 2017 Oct 19;5:e3933. doi: 10.7717/peerj.3933

Figure 4. Nicotine pre-treatment inhibits PARP-1 and caspase-3 cleavage in MPP+-treated SH-SY5Y neuroblastoma cells.

Figure 4

(A) Western blot analysis showing that cleaved caspase-3 decreased in SH-SY5Y cells pre-treated with nicotine before MPP+ treatment, as compared to those treated with MPP+ only. α-Tubulin was used as a loading control. (B and C) Densitometric analysis of expression levels of full length (B) and cleaved (C) caspase-3. The expression level of caspase-3 was defined as the ratio of the intensity of caspase-3: α-Tubulin, and was normalized as percentage of the control group, **p < 0.01, n = 3, two-way ANOVA. (D) Western blot analysis showing that cleaved PARP-1 was decreased in SH-SY5Y cells pre-treated with nicotine before MPP+ treatment, as compared to those treated with MPP+ only. α-Tubulin was used as a loading control. (E and F) Densitometric analysis of the intensity of expression levels of full length (E) and cleaved (F) PARP-1. The expression level of PARP-1 was defined as the ratio of the intensity of PARP-1: α-Tubulin, and was normalized as percentage of the control group, ***p < 0.001, n = 3, two-way ANOVA. All data are shown as mean ± SEM.