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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Cell Signal. 2017 Sep 28;40:222–229. doi: 10.1016/j.cellsig.2017.09.021

Figure 3. FGF2 inhibition of GC-B is rapidly reversible.

Figure 3

GC activity was measured in membranes prepared from whole RCS cells treated with 1 μg/ml heparin and 100 ng/ml FGF2 for 15 min, washed twice, and incubated with fresh cell culture medium at 37°C for the periods of time shown before crude membranes were prepared and assayed for GC activity in the presence of 1 μM CNP. Each point represents the average of duplicate determinations from five separate experiments such that n = 5 for each point, where vertical error bars within the symbols represent the standard error.