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In vitro enzyme assays confirm that PDE4BT407A is less sensitive to BC54 and rolipram as compared to wild type PDE4B. In vitro enzyme assays using the catalytic domains of wild type PDE4B and PDE4BT407A expressed and purified from E. coli. Values represent averages of inhibition ± S.E.M. from three independent assays carried out at 500nM cAMP substrate concentration.
