Figure 1.

DNA Binding by the Ycg1–Brn1 Condensin Subcomplex

(A) Schematic representation of the five-subunit condensin complex.

(B) Ct His₆-TEV-Ycs4_3–1222, His₆-TEV-Ycg1_24–1006, and GST-HRV3C-Brn1_336–714 proteins or equimolar combinations of the individually purified proteins were analyzed by SDS-PAGE and Coomassie staining (left panel) or used for EMSA of a 6-fluorescein-amidite (FAM)-labeled 35-bp dsDNA substrate (0.2 μM, right panel).

(C) ITC curve of the copurified Ct Ycg1_24–1006–His₆-TEV-Brn1_515–634 subcomplex binding to a 25-bp dsDNA.

See also Figure S1.