DNA Binding by the Ycg1–Brn1 Condensin Subcomplex
(A) Schematic representation of the five-subunit condensin complex.
(B) Ct His6-TEV-Ycs43–1222, His6-TEV-Ycg124–1006, and GST-HRV3C-Brn1336–714 proteins or equimolar combinations of the individually purified proteins were analyzed by SDS-PAGE and Coomassie staining (left panel) or used for EMSA of a 6-fluorescein-amidite (FAM)-labeled 35-bp dsDNA substrate (0.2 μM, right panel).
(C) ITC curve of the copurified Ct Ycg124–1006–His6-TEV-Brn1515–634 subcomplex binding to a 25-bp dsDNA.
See also Figure S1.