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. 2017 Oct 19;171(3):588–600.e24. doi: 10.1016/j.cell.2017.09.008

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© 2017 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S1 — Condensin Subunits and Protein Domains Required for DNA Binding, Related to Figure 1

(A) EMSA with 6-FAM labeled 35-bp dsDNA (0.2 μM) and copurified Ct Ycg1_24-1006- His₆-TEV-Brn1_515-634 and Ct Ycs4_3-1222–Ycg1_24-1006–His₆-TEV-Brn1_225-634 subcomplexes. Protein preparations used for EMSA are shown after SDS-PAGE and Coomassie staining.

(B) EMSA with Ct Ycs4, Ct Ycg1 proteins or copurified Ct Ycs4–Brn1_225-512 or Ct Ycg1–Brn1_515-634 subcomplexes as in (A).

(C) EMSA with copurified Ct Ycg1–His₆-TEV-Brn1_515-634 subcomplexes containing truncated versions of Ct Ycg1 (Ycg1_24-1006, Ycg1_78-1006, Ycg1_24-823, Ycg1_24-883, Ycg1_24-934, Ycg1_24-982) as in (A). Cartoons indicate truncations of Ycg1 secondary structure elements.

(D) EMSA with copurified Ct Ycg1_24-1006– His₆-TEV-Brn1 subcomplexes containing truncated versions of Ct Brn1 (Brn1_515-634, Brn1_549-634, Brn1_572-634, Brn1_515-601) as in (A). Cartoons indicate truncations of Brn1 secondary structure elements.