Condensin Subunits and Protein Domains Required for DNA Binding, Related to Figure 1
(A) EMSA with 6-FAM labeled 35-bp dsDNA (0.2 μM) and copurified Ct Ycg124-1006- His6-TEV-Brn1515-634 and Ct Ycs43-1222–Ycg124-1006–His6-TEV-Brn1225-634 subcomplexes. Protein preparations used for EMSA are shown after SDS-PAGE and Coomassie staining.
(B) EMSA with Ct Ycs4, Ct Ycg1 proteins or copurified Ct Ycs4–Brn1225-512 or Ct Ycg1–Brn1515-634 subcomplexes as in (A).
(C) EMSA with copurified Ct Ycg1–His6-TEV-Brn1515-634 subcomplexes containing truncated versions of Ct Ycg1 (Ycg124-1006, Ycg178-1006, Ycg124-823, Ycg124-883, Ycg124-934, Ycg124-982) as in (A). Cartoons indicate truncations of Ycg1 secondary structure elements.
(D) EMSA with copurified Ct Ycg124-1006– His6-TEV-Brn1 subcomplexes containing truncated versions of Ct Brn1 (Brn1515-634, Brn1549-634, Brn1572-634, Brn1515-601) as in (A). Cartoons indicate truncations of Brn1 secondary structure elements.