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. 2017 Oct 18;7:443. doi: 10.3389/fcimb.2017.00443

Figure 4.

Figure 4

Effect of up-regulation of IL-13Rα1 via lentivirus transfection. Ana-1 cells were infected with lentivirus or empty vector for 96 h except control group. The expression of IL-13Rα1 was increased significantly with lentivirus transfection. (A) Effect of lentivirus transfection: 96 h after the ana-1 cells were infected by lentivirus, the cells were observed in the fluorescence, in control light and in both the fluorescence and control light respectively. (B) Effect of lentivirus transfection on IL-13Rα1 mRNA levels detected by real-time qPCR. *p < 0.01 compared with the empty and control group (n = 3, student's t-test). (C) Effect of lentivirus transfection on IL-13Rα1 protein abundance detected by western blot assay. *p < 0.01 compared with the empty and control group (n = 3, student's t-test). Data are shown with mean with SD.