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. 2017 Oct 18;7:443. doi: 10.3389/fcimb.2017.00443

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Copyright © 2017 Li, Chen, Dang, Wang, Shang, Ma, Wang, Zhang, Luo, Li and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of down-regulation of IL-13Rα1 via siRNA transfection. The expression of IL-13Rα1 was decreased significantly with siRNA transfection. Ana-1 cells were infected with IL-13Rα1 siRNA or empty vector for 48 h except control group. (A) Effect of siRNA transfection: 48 h after the ana-1 cells were infected, the cells were observed in the fluorescence, in control light and in both the fluorescence and control light respectively. (B) Effect of siRNA transfection on IL-13Rα1 mRNA levels detected by real-time qPCR. ^*p < 0.01 compared with the empty and control group (n = 3, student's t-test). (C) Effect of siRNA transfection on IL-13Rα1 protein abundance detected by western blot assay. ^*p < 0.01 compared with the empty and control group (n = 3, student's t-test). Data are shown with mean with SD.