Figure 3.

Phagocytosis of Curvularia lunata (A) and Aspergillus fumigatus (B,C) conidia by THP-1 monocytes. THP-1 cells and conidia were stained with CellMask Deep Red Plasma Membrane Stain and Alexa Fluor 488 carboxylic acid, succinimidyl ester, respectively. Number of the Curvularia conidia and cells were set to maintain an E:T ratio of 20:1 (A), while for A. fumigatus E:T ratio was 1:2 (B) or 20:1 (C). Monocytes were identified by detecting fluorescence intensity on channel 11 (Intensity_MC_CH_11) while channel 2 (Intensity_MC_CH_2) was used to detect the conidia. Cells and conidia were co-incubated for 1 h. Fluorescent micrographs showing conidia (green border) and THP-1 cells alone (red border) and in interaction (i.e., phagocytosis or attachment) (yellow border) were recorded during the imaging flow cytometry.