Figure 4.

Relative transcript levels of pro- and anti-inflammatory cytokines, activation and antigen presentation related genes. THP-1 monocytes were confronted with Curvularia strains for 3 and 24 h. After total RNA extraction from the monocytes, cDNA synthesis, and quantitative reverse transcription PCR were carried out. Relative transcript levels were calculated by the 2^−ΔΔCt method. Presented values are averages of the results of three independent experiments; error bars represent SDs. Relative transcript values followed by * significantly differed from the control (taken as 1), according to the paired t-test (p < 0.05).