Figure 5.

Quantity of produced pro- and anti-inflammatory cytokines (pg/ml) after interaction with Curvularia strains for 3 and 24 h. THP-1 cells were interacted with the fungal conidia in an E:T ratio of 20:1. In case of the control, monocytes were incubated without the fungi. Concentrations of IL-6, IL-8, IL-10, and TNF-α in the culture supernatant were measured by ELISA. Cytokine titers were calculated by reference to standard curves generated by the four parameters logistic curve-fit method. Results are presented as averages from three independent experiments; error bars represent SDs.