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. 2017 Oct 18;8:2046. doi: 10.3389/fmicb.2017.02046

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Copyright © 2017 Chen, Tsai, Huang, Wu, Cheng, Hsu and Tsai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The relative accumulation of BaMV plus- and minus-strand RNA in NbCA-knockdown protoplasts. Real-time RT-PCR was used to quantify the accumulation of BaMV plus- and minus-strand RNA in Luc- and NbCA-knockdown N. benthamiana protoplasts at 24 and 48 hpi. The accumulation of BaMV RNA detected from Luc-knockdown protoplasts at 24 hpi was set to 100%. The numbers are the average accumulation of BaMV plus- and minus-strand RNA with the standard error obtained from three independent experiments; ^∗p < 0.05 by Student’s t-test.