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. 2017 Oct 18;8:2046. doi: 10.3389/fmicb.2017.02046

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Copyright © 2017 Chen, Tsai, Huang, Wu, Cheng, Hsu and Tsai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of NbCA in N. benthamiana cells. The pEpyon vector and NbCA-Orange fluorescent protein (NbCA-OFP) constructs were transiently expressed on N. benthamiana leaves by agroinfiltration for 3 days without (A) or with (B) inoculation of BaMV vector carrying a GFP reporter. NbCA-OFP is labeled in cyan and chloroplast is in red. BaMV infection carrying GFP is in green. Images were taken under an Olympus Fluoview FV1000 Confocal Microscope with 488, 543, and 633 nm laser excitations for GFP, OFP, and autofluorescence, respectively. Scale bar: 40 μm.