Fig. 5.

Cyclic AC253 brain uptake correlates with the expression of amylin receptor levels in the brain (A) Brain images from wild-type (Wt), heterozygous CTR knockdown (het CTR, 50% depletion of amylin receptors), or TgCRND8 (that overexpress amylin receptors) mice 2 hours after receiving equimolar intraperitoneal (ip) injections of Cy5-cAC253 (0.1 mmol in 200 μL saline). (B) Quantification of data for brain accumulation of Cy5-cAC253 in the three distinct mouse genotypes. (n = 5 in each group, ^∗P < .05, ^∗∗P < .01). (C) Brain sections from the three mouse genotypes showing fluorescent labeling through the hippocampus after a single ip injection of Cy5-labeled cAC253 (scale bar = 100 μm). Abbreviation: DAPI, 4',6-diamidino-2-phenylindole.