Table 2.

Immunohistochemistry results of NF-E2-related factor 2 and heme-oxygenase-1 (HO-1) in cerebral cortex and hippocampus. There was significant difference between sham group and each model group (P<0.05); expression of NF-E2-related factor 2 and heme-oxygenase-1 protein had significant increase in model group

Group	Nrf2 in cortex	Nrf2 in hippocampus	HO-1 in cortex	HO-1 in hippocampus
Sham	0.28±0.07	0.35±0.06	5.23±0.77	4.08±0.96
Normothermia group 15 min	0.62±0.05	0.65±0.07	10.47±1.93	9.23±2.51
Mild hypothermia before ROSC 15 min	0.74±0.19	0.70±0.11	14.88±1.90	13.90±2.60
Mild hypothermia after ROSC 15 min	0.81±0.15	0.63±0.05	13.19±1.86	13.30±1.29
Normothermia group 30 min	0.67±0.16	0.58±0.16	9.66±1.60	10.33±1.73
Mild hypothermia before ROSC 30 min	0.95±0.11	0.84±0.07	12.37±2.13	16.98±3.63
Mild hypothermia after ROSC 30 min	0.80±0.10	0.81±0.13	14.53±2.66	13.38±3.38
Normothermia group 60 min	0.56±0.12	0.57±0.14	10.01±0.90	10.45±2.14
Mild hypothermia before ROSC 60 min	1.06±0.17	0.90±0.13	15.03±3.79	15.33±2.78
Mild hypothermia after ROSC 60 min	0.76±0.22	0.71±0.08	14.32±2.65	15.83±2.37
F	9.546	8.520	6.722	9.636
P	0.000	0.000	0.000	0.000

ROSC: Restoration of spontaneous circulation.