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Calycosin suppresses NF-κB signal pathway.
Total colon tissue lysates were prepared for detecting the activation states of IκBα, p65 and IKKα/β by Western blot (A). The action was used as protein loading control. The relative density of the signaling band on Western blot was compared with the actin band in each group (B). The data based on 3 independent experiments represent means ± SD, and −P < 0.05 compared with dextran sulfate sodium (DSS)+vehicle group
