Figure 4.

Apatinib induced apoptosis and autophagy through inhibition of AKT/mTOR pathway

(a) HCT116 and SW480 cells were treated with apatinib (0, 20, 40 μM, respectively) for 24 hr, and the alterations of phosphorylated AKT and mTOR were detected. (b) HCT116 and SW480 cells were treated with apatinib (0, 20, 40 μM, respectively) for 24 hr, and the protein level of LC3 was detected. GAPDH was included as a loading control