Table 1.
Summary of sequence coverage, number of peptides and PSMs for analysis of GLI1 tryptic peptides by mass spectrometry.
| Sample name | Sample # | Runa | Coverage (%)b | # Peptides | PSMc |
|---|---|---|---|---|---|
| GLI1 | 1 | 1 | 57.73 | 114 | 386 |
| GLI1 | 1 | 2 | 48.35 | 76 | 279 |
| GLI1 + DYRK1A + ATP | 2 | 1 | 74.39 | 144 | 720 |
| GLI1 + DYRK1A + ATP | 2 | 2 | 72.22 | 143 | 692 |
| GLI1 + DYRK1A - ATP | 3 | 1 | 77.69 | 161 | 762 |
| GLI1 + DYRK1A - ATP | 3 | 2 | 61.98 | 118 | 543 |
a
Each gel slice was divided into 2 pieces, treated and analyzed separately.
b
Coverage (%) refers to the % coverage of the entire GLI1 protein.
c
PSMs are the number of spectra that matched peptides and is an approximate estimate of amount.