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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Am Chem Soc. 2017 Feb 21;139(8):2994–3004. doi: 10.1021/jacs.6b11011

Figure 5.

Figure 5

Enet vs. pH diagrams for α3Y and the α3FnY proteins. Solid dots represent Enet values obtained by PFV. Grey dots represent Enet collected from α3(3,5)F2Y using the following conditions: 70 μM protein, 20 mM APB, 75 mM KCl; pH adjusted with 1 M NaOH; step potential 0.15 mV, SW pulse amplitude 25 mV, SW frequency 190 Hz, and temperature 25 °C.