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FBPase inhibition by S-nitrosylation of Cys153. A, FBPase activity assays of the FBPase mutants C153S and C173S C178S treated or non-treated with GSNO. B, Effect of GSNO treatment on the affinity of C173S C178S for the fructose-1,6-bisphosphate (FBP). Experimental conditions of panels A and B were the same as shown in the legend of Fig. 3. C, Activity recovery of GSNO-inhibited C173S C178S after incubation (30 min at room temperature) with reduced glutathione (GSH).
