Table 1.

Characteristics of a good marker and of the Immunoscore [8]

Characteristics
Routine	Technic to be performed by pathologist using bright field and precise cell evaluation
Feasible	Established pathology technics, using 2 regular whole slide FFPE section
Inexpensive	Automatized immunohistochemistry
Rapid	2 simple staining less costly than complicated molecular techniques
Robust	Autostainers, scanner, and digital pathology reduce the time to perform an Immunoscore
Reproducible	Two strong membrane staining, with no background, allowing the numeration of individual cells
Quantitative	Inter-observers variability is removed by the use of digital pathology, taking into account cell location and counts
Standardized	Standardized operating procedure should be performed to insure reproducibility and worldwide comparisons
Pathology-based	Necessity of pathologist expertise to validate cell type, cell location, and cell counts performed by digital pathology
Powerful	The Immunoscore has a prognostic value highly significant even in Cox multivariate including TNM classification