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. 2017 Oct 23;18:488. doi: 10.1186/s13063-017-2246-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — System of trial gas inhalation. The trial gas will be inhaled through this system. Trial gas will be supplied from a cylinder with constant flow, and it will be mixed with oxygen from the mechanical ventilator (SERVO-s®; MAQUET Critical Care AB) at the inspiratory duct. The cylinder is filled with 4% H₂ and 96% nitrogen for the hydrogen group and 100% nitrogen for the control group. A tidal volume and fraction of inspired oxygen meter will be used during trial gas inhalation to monitor ventilation