Fig. 4.

Adiponectin ablation reduces the thermogenic capacity of mitochondria in BAT. BAT from male 10-month old WT and Adipoq ^−/− mice were collected after a 6 h of 4 °C cold exposure. a Quantification of mitochondrial density. b Expression of mitochondrial dynamic genes. c, d Expression of putative adiponectin-mediated thermogenic regulators. e Representative Western blots of AMPK activation in BAT from WT and Adipoq ^−/− mice. p-AMPK for phosphorylated AMPA; t-AMPK for total AMPK. f Expression of SIRT1, a downstream target of AMPK. n = 6. *P < 0.05, **P < 0.001 WT vs. Adipoq ^−/− mice