Fig. 3. Effects of Ell3 suppression on the in vivo wound healing activity of ADSC-CM. CM collected from siNS- or siEll3-transfected ADSCs was applied to mouse wounds every 24 hours for 7 days.

Serum-free media (SFM) was applied as a negative control. (A) The backs of mice were wounded by punches. (B) Wound areas to which SFM and CM collected from siNS- and siEll3-transfected ADSCs were applied 7 days after treatment. (C) The wound area was quantitatively analyzed on the indicated days after treatment. (D) Sections of paraffin-blocked wounded tissues were stained with hematoxylin and eosin (H&E). The arrows indicate E (epi-dermis), D (dermis), and I (inflammation). (E) Relative expression of IL-1 and IL-6 transcripts in ADSCs transfected with siNS- or siEll3- for 24 hrs.