TABLE 2.
Cervid transgenic mouse bioassay: inocula and Western blotting and RT-QuIC analysis
| Muntjac dam disease status and identification no(s). | Inoculum |
Clinical TSE diseasea |
Western blotting of brainc | RT-QuIC assayc |
|||
|---|---|---|---|---|---|---|---|
| Tissue or fluid source (n) | Amt and route | No. of positive mice/total no. of mice | Time to disease (avg dpi ± SD) | Brain | Spleen | ||
| CWD positive | |||||||
| 45 | Uterus (8) | 30 μl i.c. of 10% homogenate | 8/8 | 302.6 ± 45.8 | 8/8 | 8/8 | 7/8 |
| Placentome 1 (6) | 30 μl i.c. of 10% homogenate | 0/6 | 500 | 0/6 | 0/6 | 1/6 | |
| 47 | Placentomes 1 and 5 (8) | 30 μl i.c. of 10% homogenate | 7/8 | 198.3 ± 45.4 | 6/8 | 8/8 | 8/8 |
| Amniotic fluid (9) | 30 μl i.c. | 1/9 | 320 | 0/9 | 1/9 | 2/9 | |
| Allantoic fluid (9) | 30 μl i.c. | 0/9 | 0/9 | 0/9 | 0/9 | ||
| CWD negative | |||||||
| 64 | Uterus (9) | 30 μl i.c. of 10% homogenate | 0/24b | 0/24 | 0/9 | 0/9 | |
| Placentome 1 (8) | 30 μl i.c. of 10% homogenate | 0/8 | 0/8 | ||||
| 64 and 107 | Amniotic and allantoic fluid (7) | 30 μl i.c. | 0/7 | 0/7 | |||
a
Clinical disease, demonstrated by lethargy, stiff tail, circling, and hind limb ataxia.
b
An age-matched negative-control mouse was harvested with each mouse euthanized with clinical TSE disease or at study termination at 343 days postinfection (dpi).
c
Values are the number of positive mice/total number of mice tested.