TABLE 1.
Analysis of GC content and CpG dinucleotide frequencies in rB/HPIV3-RSV F ORFsa
| ORFb | No. of nucleotides | % GC contentc | No. of CpG dinucleotidesd | CpG dinucleotide frequencye (%) |
|---|---|---|---|---|
| Non-HEK/non-opt | 1,725 | 34.7 | 12 | 0.70 |
| GA | 1,725 | 57.6 | 100 | 5.80 |
| GS | 1,725 | 49.2 | 66 | 3.83 |
| N | 1,548 | 41.3 | 28 | 1.81 |
| P | 1,791 | 41.1 | 31 | 1.73 |
| M | 1,056 | 40.0 | 13 | 1.23 |
| F | 1,620 | 35.6 | 17 | 1.05 |
| HN | 1,719 | 36.1 | 16 | 0.93 |
| L | 6,702 | 33.4 | 68 | 1.01 |
a
Sequences were analyzed with the GeneQuest module in the Lasergene 13 software package (DNASTAR).
b
Coding sequences of ORFs of RSV F (non-HEK/non-opt, GA, and GS) and rB/HPIV3 structural proteins (N, P, M, F, HN, and L) as illustrated in Fig. 1 were analyzed.
c
Percentage of G and C nucleotides in the ORF.
d
Number of CpG dinucleotides in the ORF.
e
Frequency of CpG dinucleotides in the ORF.