FIG 1.

Localization of segment 7 mRNA in infected and transfected cells. 293T cells were infected or mock infected with Cambridge PR8 at an MOI of 5 and fixed at 6 h p.i. (A) or transfected with plasmids to reconstitute RNPs (3PNP) containing segment 7 vRNA or with a negative-control set lacking PB2 (2PNP) and fixed 24 h later before. Cells were then stained for positive-sense segment 7 RNA by FISH (red) or for DNA (4′,6′-diamidino-2-phenylindole; blue) and imaged by confocal microscopy (B). Single optical slices are shown. Scale bar, 10 μm.