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Interaction between NXF1 and NS1. 293T cells were transfected with plasmids encoding GFP or GFP-NXF1 and 48 h later either mock infected or infected with the indicated NS1 mutant viruses. Cells were harvested at 6 h p.i., and cell lysates were examined by Western blotting for the indicated proteins before (Total) or after (Bound) fractionation over GFP-Trap agarose.
