Fig. S5.

(A) USA300 cells were grown at the temperatures shown (30 °C, 37 °C, and 42 °C) in TSB alone or in TSB supplemented with the indicated LA concentration. Whole-cell lysates were analyzed for EsxA protein by Western blot. (B) USA300 WT or USA300 cells expressing crtMN from the pMK4 plasmid were grown in TSB alone or in TSB supplemented with 10 µM LA. Membrane fluidity was measure using the pyrenedecanoic acid fluorescence method. The increasing ratio of fluorescence at 460 nm and 405 nm reflects increased membrane fluidity. (C) USA300 WT, arlS-, agrB-, and saeRS-mutant strains were grown in TSB alone or in TSB supplemented with 10 µM LA. Whole-cell lysates were assessed for EsxA protein by Western blot. The data represent the average values of three independent experiments; error bars represent the SE.