Fig. 3.

A quantitative analysis of silencing loss dynamics in single cells. (A) Dynamics of silencing loss in representative single cells with different life spans. For each cell, (Top) time-lapse images for the cell have been shown with (Bottom) the fluorescence time trace throughout its life span. Vertical dashed line represents each division time of the cell, in which the distance between two adjacent dashed lines indicates the cell cycle length. Reporter fluorescence is normalized to the baseline level. (B) A schematic illustrates the dissection of two phases based on the silencing loss dynamics. The rise times of early sporadic and final sustained silencing loss are defined as t₁ and t₂, respectively. (C) Scatter plots showing the relationships between the length of (Left) Intermittent Phase or (Right) Sustained Phase and life span at the single-cell level. Single-cell data are from Fig. 2A. Each circle represents a single cell. Correlation coefficient (R) is calculated and shown. (D) Bar graph showing the average durations of (blue) early sporadic and (red) final sustained silencing loss.