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. 2017 Oct 3;114(42):11253–11258. doi: 10.1073/pnas.1703379114

Fig. 5.

Fig. 5.

Perturbations of silencing dynamics shorten life span. (A) Dynamics of silencing loss in sir2Δ cells. (Left) Single-cell color map trajectories of reporter fluorescence. Each row represents the time trace of a single cell throughout its life span. Color bar is identical to Fig. 2. Cells are sorted based on their RLS. The red dashed line represents the average length of Sustained Phase in WT cells, defined in Fig. 3B. (Right) Representative single-cell time traces with different life spans. Dynamics of silencing loss in WT cells are shown in response to chemical perturbations. (B) A 1,000-min 5 mM NAM pulse. (Inset) Expression of RNR3-mCherry in response to a 1,000-min 5 mM NAM pulse at indicated times. (C) A 240-min 5 mM NAM pulse. Inset shows the expression of RNR3-mCherry in response to a 240-min 5 mM NAM pulse at indicated times. (D) A constant treatment of 2.5 mM NA. For each condition, (Top) a schematic illustrating the time-based drug treatment has been shown with (Bottom) the corresponding single-cell color map trajectories of reporter fluorescence.