Fig. S10.

Cross-correlation of fluorescence and force trajectories was facilitated by observing two-photon fluorescence from the optical trapping laser oscillated through the fluorescence collection region. A trajectory that oscillates for many cycles with a distinct change in oscillation pattern near the end allows for the time delay and time step to be fitted. (A) Two-photon fluorescence collected using the EMCCD and laser position derived from a piezoelectric tip/tilt actuator encoder. (B and C) Overlay of both signals at (B) early and (C) late times (gray regions in A), using the EMCCD software exposure time (5.095 ms). (D and E) Overlay of both signals at (D) early and (E) late times, using the fitted exposure time (5.0886 ms).