Fig. S10.

Examining the effects of RNAi factors knockdown. The levels of AGO1, AGO2, and HP1γ mRNAs (A, C, and E) as quantified by RT-PCR [P(AGO) = 0.006, P(AGO2) = 0.005, P(HP1γ) = 0.001; *P < 0.05; **P < 0.01], or (B, D, and F) as seen in immunofluorescence, following siRNA treatment for 72 h. For HP1, a pan-HP1 antibody was used. In immunofluorescence, AGO1 KD was seen as cytoplasmic reduction; for AGO2, KD was mostly seen in the nucleus; and, for HP1γ, KD was seen in the nucleus (but reduction of signal cannot reach full levels, since the antibody is a pan-HP1 Ab). (G) E6 cells that express the sh-GFP were induced by dox for 24 h. A reduction in E6 transcription site intensity was observed by RNA FISH (gray) when the sh-GFP was induced, in comparison with no drop in intensity when AGO2 siRNA KD took place in parallel. Endogenous AGO2 protein levels are seen in green. (Scale bar, 20 μm.)