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SGLs inhibit NF-κB activation induced by various TLR2 agonists. HEK-TLR2 cells were stimulated by M. tuberculosis lipoproteins (A), Pam3CSK4 (B), FSL-1 (C), or M. tuberculosis lipomannan (D) in the presence or not of Ac4SGL or Pam2SGL at the indicated concentrations. After 16 h, NF-κB activation was determined by measuring alkaline phosphatase activity in the culture supernatant and reading OD at 630 nm. Data show mean ± SEM. n.i., not induced.
